Analysis of carbohydrates content in the plant components of antidiabetic herbal mixture by GC-MS

Medicinal plants and their combinations due to the wide range of biologically active substances can influence on various links pathogenetic mechanism development diabetes mellitus its complications. One such is an antidiabetic herbal mixture ( Urticae folia , Rosae frucrus Myrtilli Menthae Taraxaci radices ) with established hypoglycemic, hypolipidemic, antioxidant, hepatoprotective, pancreatoprotective activity in previous pharmacological study vivo. Thus, aim this was identify establish content carbohydrates free bound form plant components mixture. The were separated by gas chromatography-mass spectrometry after conversion into volatile derivatives as aldononitrile acetate. monomeric composition polysaccharides studied hydrolysis monosaccharides polyalcohols. results quantitative showed that predominant carbohydrate saccharose L -fructose -glucose . Concerning determination monomers hydrolysis, prevailed all chromatographic revealed a number polyalcohols are important for treatment prevention progression complications, namely, mannitol myo -inositol.